Detection of Raspberry ringspot virus Using Reverse Transcription Loop-mediated Isothermal Amplification
نویسندگان
چکیده
Raspberry ringspot virus (RpRSV), which has not occurred in Japan, infects many plants such as raspberry, gooseberry, cherry, grapevine, and strawberry. Biological assay, enzyme-linked immunosorbent assay (ELISA), and reverse transcription polymerase chain reaction (RT-PCR) have been introduced for the detection of RpRSV in postentry quarantine in Japan. We studied reverse transcription loop-mediated isothermal amplification (RT-LAMP) for RpRSV detection. As a result, the detection limit in RT-LAMP that employed primers specific for RpRSV was more than 10 times as high as that in the RT-PCR. Furthermore, simple and rapid sample preparation methods were evaluated, and the virus detection time was able to be reduced compared to modified SDS‒Potassium acetate method.
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